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Science/Engineering Yeast Protocols: Methods in Cell and Molecular Biology: Ivor H. Evans

Posted on 2010-03-16




Name:Science/Engineering Yeast Protocols: Methods in Cell and Molecular Biology: Ivor H. Evans
ASIN/ISBN:0896033198
Language:English
File size:26.47 Mb
ISBN: 0896033198
Publish Date: 1996-03-01
File Type: PDF (OCR)
Pages: 433 pages
File Size: 26.47 Mb
Other Info: Humana Press
   Science/Engineering Yeast Protocols: Methods in Cell and Molecular Biology: Ivor H. Evans

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Yeast Protocols: Methods in Cell and Molecular Biology: Ivor H. Evans

Yeast Protocols contains many key techniques for studying the biology of yeasts at both the cellular and molecular levels. Working primarily from Saccharomyces cerevisiae, the expert contributors explain step-by-step how to successfully isolate, identify, and culture yeasts; the secrets of meiotic mapping; how to use PFGE in karyotyping and gene localization; the methods for purification and analysis of various cell components; and the construction and exploitation of genomic DNA clone banks. They also cover the latest methods for chromosome engineering, insertional mutagenesis by Ty elements, mRNA abundance and half-life measurements, the use of reporter gene systems, genotoxicity testing, and more. Yeast Protocols follows the widely applauded Humana Methods in Molecular Biology style: brief introductions putting the particular method in context, comprehensive lists of materials, cookbook style instructions, and troubleshooting notes to avoid common pitfalls and solve problems. The techniques can be used with confidence and success by both inexperienced newcomers and established researchers.

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