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Study Laser Capture Microdissection: Methods and Protocols

Posted on 2010-03-15




Name:Study Laser Capture Microdissection: Methods and Protocols
ASIN/ISBN:1588292606
Language:English
File size:6 Mb
ISBN: 1588292606
Pages: 310 pages
Publish Date: December 23, 2004
File Type: PDF
File Size: 6 Mb
Other Info: Humana Press; 1 edition
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Laser Capture Microdissection: Methods and Protocols

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip? microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI? approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.

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