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Technical Enzyme immunoassays:From Concept To Product Development

Posted on 2010-03-17




Name:Technical Enzyme immunoassays:From Concept To Product Development
ASIN/ISBN:0412056011
Author:S. S. Deshpande
Publisher:Springer
Language:English
Pages:464
File size:1 Mb
   Technical Enzyme immunoassays:From Concept To Product Development

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  • Author: S. S. Deshpande
  • Publisher: Springer
  • Pages: 464

This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.

Contents

Introduction 3
antigen , antibodies , bioassays
Biochemistry Structure and Function 24
immunoglobulins , antigen , immunogens
AntigenAntibody Reactions 52
Scatchard plot , immunoassay , hydrogen bonds
Conjugation Techniques 72
cross-linking , thiol , nucleophilic
Antibody Production 120
monoclonal antibodies , theophylline , codeine
Enzymes and Signal Amplification Systems 155
avidin , lysozyme , alkaline phosphatase
more »

Separation and SolidPhase Systems 193
microtiter plates , adsorption , hydrophobic
Immunoassay Classification 231
immunoradiometric assay , equilibrium constant , chemiluminescent
Heterogeneous Enzyme Immunoassays 240
liposomes , CEDIA , zyme
Assay Development Evaluation and Validation 275
polystyrene , adsorption , analyte concentration
Reagent Formulations and Shelf Life Evaluation 360
eutectic , freeze-drying , glass transition temperature
Data Analysis 401
calibration curve , tolerance interval , analyte concentration
Documentation Registration and Diagnostics StartUps 426
quality system , GMPs , Quantitative market research
453
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