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Science/Engineering Two-Dimensional Electrophoresis Protocols (Methods in Molecular Biology)

Posted on 2010-03-16




Name:Science/Engineering Two-Dimensional Electrophoresis Protocols (Methods in Molecular Biology)
ASIN/ISBN:1588299376
Language:English
File size:5.2 Mb
Publish Date: 2009
ISBN: 1588299376
Pages: 560 pages
File Type: PDF
File Size: 5,2 MB
Other Info: Humana Press
   Science/Engineering Two-Dimensional Electrophoresis Protocols (Methods in Molecular Biology)

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David Sheehan, Raymond Tyther, "Two-Dimensional Electrophoresis Protocols (Methods in Molecular Biology)"

Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists.

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