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Medical Trinucleotide Repeat Protocols (Methods in Molecular Biology)

Posted on 2010-03-17




Name:Medical Trinucleotide Repeat Protocols (Methods in Molecular Biology)
ASIN/ISBN:1588292436
Author:Yoshinori Kohwi
Publisher:Humana Press
Publish Date:June 18, 2004
Language:English
Pages:360
File size:2.5 Mb
   Medical Trinucleotide Repeat Protocols (Methods in Molecular Biology)

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  • Author: Yoshinori Kohwi
  • Publisher: Humana Press
  • Publish Date: June 18, 2004
  • ISBN: 1588292436
  • Pages: 360
Established leaders in trinucleotide repeat disease describe in step-by-step detail their best techniques for studying trinucleotide pathology at the molecular level. The protocols cover a variety of targets, ranging from DNA and RNA to proteins and whole animals, and focus not only on causal genes, but also on their consequent affected products, such as transcription factors, neurotransmitter receptors, proteasomes, and mitochondria/oxidation damage. Experimental systems employed include E. coli, yeast, C. elegans, mouse, and generally take a clinical point of view. The authors utilize a wide range of techniques, including gel electrophoresis, quantitative RT-PCR, immunological analysis, antibody usage and its applications, receptor assays using radioisotope handling, gene delivery by virus, brain cell and organotypic cultures, gender dependency, and neuron structure analysis.

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