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Technical Transmembrane Transporters
Posted on 2010-04-14
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More Michael W. Quick, "Transmembrane Transporters (Receptor Biochemistry and Methodology)" A must-have far-reaching text that provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be used in cutting edge therapies. Each chapter begins with an overview of the importnat biological questions presently being considered in their field, then presents scientific approaches to address these questions. In explaining approaches, the authors cover bench-top protocols, conceptual frameworks, data obtained, and pitfalls common to the techniques. Transmembrane transporters represent a class of proteins designed to move molecules across biological membranes. Likely present in all subcellular compartments of all cells of all organisms, transporters perform a myriad of diverse functions, such as the acquisition of nutrients, regulation of metabolite concentrations, removal of toxins, maintenance of ionic and chemical gradients, and secretion of macromolecules and nucleic acids. Transmembrane Transporters provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be used in cutting-edge therapies. The book's authors begin each chapter with an overview of the important biological questions presently being considered in their field, then present scientific approaches to address these questions. In explaining approaches, the authors cover experimental approaches, conceptual frameworks, data obtained, and pitfalls common to the techniques. Easy-to-reference protocol sections offer flow diagrams and illustrations to better elucidate new and unfamiliar techniques. Coverage in Transmembrane Transporters includes: · Informatics · Expression strategies · High-throughput screening · Molecular genetics · Structural analyses, such as NMR, crystallization, freeze fracture, and chimeras · Functional analyses, such as imaging, electrophysiology, and microdialysis Transmembrane Transporters provides readers new to the field with an accessible introduction to the wide variety of transporter research approaches, while offering practicing researchers a thorough analysis of the cutting-edge work currently being performed. This text proves a compelling resource for students and professionals in neuroscience, molecular biology, biochemistry, and biophysics. Links or
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