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siRNA and miRNA Gene Silencing: From Bench to Bedside

Posted on 2010-08-15




Name:siRNA and miRNA Gene Silencing: From Bench to Bedside
ASIN/ISBN:1603275460
File size:5 Mb
Publisher: Humana Press
Publish Date: 2008
File Type: PDF
Pages: 474 pages
ISBN: 1603275460
Other Info: 5Mb
   siRNA and miRNA Gene Silencing: From Bench to Bedside

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RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In , expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA’s unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection.

Comprehensive and cutting-edge, offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.

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