RTPCRPr0t2nd rar (1607616289)

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RT-PCR Protocols, 2nd Ed

Posted on 2010-04-13

Name:	RT-PCR Protocols, 2nd Ed
ASIN/ISBN:	1607616289
Language:	English
File size:	14 Mb
ISBN:	1607616289
Publish Date:	edition 2010
File Type:	PDF
Pages:	330 pages
File Size:	14 mb
Other Info:	Humana Press

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Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCRnested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducibleprotocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Hotfile Links :-

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