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Science/Engineering RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals

Posted on 2010-04-28




Name:Science/Engineering RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals
ASIN/ISBN:3527310207
Author:Ute Schepers
Publisher:Wiley-VCH (2005)
Pages:Hardcover, 336 pages
File size:5 Mb
   Science/Engineering RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals


Author: Ute Schepers


Publisher: Wiley-VCH (2005)


Binding: Hardcover, 336 pages


pricer: $170.00


ISBN-10: 3527310207


editorialreviews

This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn:



  • How RNAi works in worms, flies and mammals

  • How to design the most efficient RNAi constructs

  • How to achieve transient, stable and conditional RNAi in cell cultures

  • How to determine the efficiency of an RNAi experiment

  • and how to use RNAi for gene therapy


All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.




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