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Science/Engineering Plant Hormones: Methods and Protocols

Posted on 2010-03-15




Name:Science/Engineering Plant Hormones: Methods and Protocols
ASIN/ISBN:1934115320
Language:English
File size:3 Mb
Publisher: Humana Press
Pages: 152
Publish Date: 2008-11-21
ISBN: 1934115320
File Type: PDF
File Size: 3 MB
   Science/Engineering Plant Hormones: Methods and Protocols

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Plant Hormones: Methods and Protocols

The last ten years have witnessed a monumental increase in our understanding of plant hormones. A decade ago, scientists were forced to rely on vague ideas about hormone action; today, these have been replaced by detailed molecular models. Given the rapid increase in our understanding of plant hormone biology, this comprehensive review could not have come at a better time. In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approaches for understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls

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