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Science/Engineering Parasite Genomics Protocols

Posted on 2010-03-16




Name:Science/Engineering Parasite Genomics Protocols
ASIN/ISBN:158829062X
Language:English
File size:6.2 Mb
Publish Date: 2004-05-17
ISBN: 158829062X
Pages: 472 pages
File Type: PDF
File Size: 6.2 MB
Other Info: Humana Press
   Science/Engineering Parasite Genomics Protocols

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Sara E. Melville "Parasite Genomics Protocols"

Pioneering bench researchers describe in detail the cutting-edge techniques they have developed for analyzing the genomes and gene products in a diverse range of protozoan and metazoan parasites. These readily reproducible techniques can be used in genomic, functional genomic, and post-genomic studies and include transfection methods and vectors for several protozoan parasites, global analysis using microarrays, gene ablation using RNA interference, gene knockout, mutagenesis, and chromosome manipulation. Some of the protocols require DNA sequence data, whereas others were developed independently of whole-genome sequence data. The protocols presented follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.

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