Book123

    Free register and download UseNet downloader, then you can FREE Download from UseNet.

    Download without Limit " Technical Molecular Cloning: A Laboratory Manual (Third Edition) [Repost] " from UseNet for FREE!

More

Joseph Sambrook, David Russell

Molecular Cloning: A Laboratory Manual (3rd Edition) [3 Vols Set]

In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques.

The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.

These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.

The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions.

The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Contents:

Chapter 1. Plasmids and Their Usefulness in Molecular Cloning

Chapter 2. Bacteriophage lambda and Its Vectors

Chapter 3. Working with Bacteriophage M13 Vectors

Chapter 4. Working with High-capacity Vectors

Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis

Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA

Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells

Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction

Chapter 9. Preparation of Radiolabeled DNA and RNA Probes

Chapter 10. Working with Synthetic Oligonucleotide Probes

Chapter 11. Preparation of cDNA Libraries and Gene Identification

Chapter 12. DNA Sequencing

Chapter 13. Mutagenesis

Chapter 14. Screening Expression Libraries

Chapter 15. Expression of Cloned Genes in Escherichia coli

Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells

Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells

Chapter 18. Protein Interaction Technologies

Appendices

Buy Book at Lowest Price on Amazon

Rating:
2.5 out of 5 by Book123