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Molecular Biology Problem Solver: A Laboratory Guide

Posted on 2010-09-21




Name:Molecular Biology Problem Solver: A Laboratory Guide
ASIN/ISBN:0471379727
Publish Date:2001
Pages:528 pages
File size:2.7 Mb
Publish Date: 2001
ISBN: 0471379727
Pages: 528 pages
File Type: PDF
File Size: 2,7 MB
Other Info: Wiley-Liss
   Molecular Biology Problem Solver: A Laboratory Guide

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Alan S. Gerstein, ""

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. asks the reader to consider crucial questions, such as:

* Have you selected the most appropriate research strategy?

* Have you identified the issues critical to your successful application of a technique?

* Are you familiar with the limitations of a given technique?

* When should common procedural rules of thumb not be applied?

* What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:

* Working safely with biological samples and radioactive materials

* DNA and RNA purification

* PCR

* Protein and nucleid acid hybridization

* Prokaryotic and eukaryotic expression systems

* Properly using and maintaining laboratory equipment

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