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Science/Engineering Essentials of Toxic Chemical Risk: Science and Society
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Science/Engineering Modern Derivatization Methods for Separation Science by: Toshimasa Toyo'oka
Posted on 2010-03-16
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More Modern Derivatization Methods for Separation Science by: Toshimasa Toyo'oka Biologically active compounds are ubiquitous in our lives. They are used for medicines, agrochemicals (e.g. pesticides and harbicides), food additives, biogenic amines and flavors. It is fairly difficult to determine them with accuracy and precision, because they usually exist in munite amounts, especially in real biological and environmental samples. The choice of a suitable method that provides good reproducibility is essential to obtain correct results. Separation analysis represented by various chromatography is recommended for the quantitation of analytes in complex matrices. Derivatization was an important technique for analysis using gas chromatography in the early stages. The main purpose of the derivatization was to add volatility to saccarides and amino acids. In this derivatization, selectivity and sensitivity were not considered. However, derivatization is the essential technique in separation sciences using thin-layer chromatography (TLC), liquid chromatography (LC) and capillary electrophoresis (CE), as well as gas chromatography (GC). For analysis by highperformance liquid chromatography (HPLC), various reagents have been developed to increase separability, selectivity and sensitivity. This is due to the development of various types of detection instruments such as ultravioletvisible (UV-VIS), fluorescence (FL), chemiluminescence (CL) and electrochemical (EC). The use of derivatization in separation sciences is mainly to improve the chromatographic properties and detection sensitivity. RS Link:
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