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Science/Engineering Matrix Metalloproteinase Protocols (Methods in Molecular Biology) by Ian M. Clark

Posted on 2010-03-16




Name:Science/Engineering Matrix Metalloproteinase Protocols (Methods in Molecular Biology) by Ian M. Clark
ASIN/ISBN:0896037339
Language:English
File size:3.4 Mb
Publisher: Humana Press
Publish Date: January 15, 2001
ISBN: 0896037339
Pages: 545
File Type: PDF
File Size: 3.4 MB
   Science/Engineering Matrix Metalloproteinase Protocols (Methods in Molecular Biology) by Ian M. Clark

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Matrix Metalloproteinase Protocols (Methods in Molecular Biology) by Ian M. Clark

Comprehensive set of proven and cutting-edge methods for studying MMPs and TIMPs. Summarizes the experience of expert laboratorians, and presents reproducible techniques for the expression and purification of MMPs and TIMPs. For researchers.

"The editor, Ian M. Clark, has done an excellent job in putting together a collection of articles that span what is now a fairly wide field. ...the content and writing are of a high standard...In chapter 3, covering structural studies and contributed by Wolfram Bode and Klaus Maskos, the authors point out the absence of specific discussions of three-dimensional structures amongst the plethora of general reviews on MMPs and TIMPs. Their contribution expertly fills that void....Indeed the 'Notes' section, at the end of each chapter, is where the benefit of much, often hard-earned, experience is to be reaped and is a valuable addition in almost every case. The last two sections, between them, cover detection of MMp and TIMP expression at both RNA and protein levels, and actively assays. Hore, particular mention must be made of the chapter dealing with zymography and reverse zymography in which Susan Hawkes and coauthors have carefully explained this technique. Their use of figures with detailed interpretation is really excellent and alone could justify the purchase of this book for many labs....this book is recommended as a reference text for all labs engaged in MMP research. There is enough variety for established research labs to find some new methods, and, for newcomers to the field, it should make working with MMPs and their inhibitors a very straightforward prospect." - TRENDS in Cell Biology

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