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Science/Engineering In vitro mutagenesis protocols: Jeff Braman

Posted on 2010-03-16




Name:Science/Engineering In vitro mutagenesis protocols: Jeff Braman
ASIN/ISBN:0896033325
Language:English
File size:24.72 Mb
ISBN: 0896033325
Publish Date: 1996-03-15
File Type: PDF (OCR)
Pages: 390 pages
File Size: 24.72 Mb
Other Info: Humana Press
   Science/Engineering In vitro mutagenesis protocols: Jeff Braman

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In vitro mutagenesis protocols: Jeff Braman

Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.

Review: Contains detailed description of protocols This book is a good experimental handbook. It contains detailed step by step instructions and important notes for each experiment; and easy to follow.

Note: Cover is from a later edition.

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