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Science/Engineering Aqueous Size-Exclusion Chromatography

Posted on 2010-03-16




Name:Science/Engineering Aqueous Size-Exclusion Chromatography
ASIN/ISBN:0444429573
Language:English
File size:15.4 Mb
Publish Date: April 1988
ISBN: 0444429573
Pages: 472 pages
File Type: PDF
File Size: 15.4 mb
Other Info: Elsevier Science Ltd
   Science/Engineering Aqueous Size-Exclusion Chromatography

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Paul L. Dubin,"Aqueous Size-Exclusion Chromatography "

The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses. These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography.



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